Heat-induced complex formation of lactoferrin analyzed by an SDS-agarose-polyacrylamide (SDS-AG-PAGE) hybrid gel

SDS Polyacrylamide Gel Electrophoresis of Proteins.

Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Common...

Unpredictable behaviour of mucins in SDS/polyacrylamide-gel electrophoresis.

Knowledge Management of 1D SDS PAGE Gel Protein Image Information

Computational management and analysis of proteomics data is providing important insights into the biological and clinical sciences. In this research study, a knowledge management strategy for 1D SDS PAGE gel protein image information has been developed and implemented in the New Vision to Protein intensity Analysis System (NeVPAS) application. This application focused on the analysis of skin mu...

Use of DNA ladders for reproducible protein fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantitative proteomics.

In proteomics, one-dimensional (1D) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is widely used for protein fractionation prior to mass spectrometric analysis to enhance the dynamic range of analysis and to improve the identification of low-abundance proteins. Such protein prefractionation works well for quantitation strategies if the proteins are labeled prior to separa...

SDS-PAGE heat-shock protein profiles of environmental Aeromonas strains.

Aeromonas microorganisms normally grow at temperatures between 5 degrees C and 45 degrees C and therefore should have high thermotolerance. Thus it was of interest to find out whether A. hydrophila, A. caviae and A. veronii biovar sobria serovars respond to abrupt temperature changes with a heat shock-like response. To this end the present study was undertaken to determine whether Aeromonas spe...